The purpose of the experiment was to observe how substrate concentration affects enzyme activity. My supposition is that the much substrate concentration thither is, the more product it will form; this is until it reaches the point of saturation, after(prenominal) that, the enzyme will be unable to produce at a faster rate. To prove this, twenty atomic number 23 trials were made, five trials for five different concentrations of substrate with the same amount of enzyme, recording the rate of production for each trial. For this experiment, hydrogen peroxide was used as the substrate, and catalase as the enzyme. Each trial was five milliliters of substrate, adding ten drops of enzyme, delay 30 secs, shaking it twice, and pouring it into a bottle where the atomic number 8 (as the product) would be recorded. The outcome of this experiment turned out as follows: the average rate of molecular type O produced for two per centum hydrogen peroxide was 0.05 percent of molecular oxygen per second; for four, six, eight and ten percent the average rate was 0.06, 0.07, 0.08, 0.10 percent of molecular oxygen per second respectively.
The results are close to being linear, with an r² value of 0.9888.
One could say the outcome is safe because it shows how by having more concentration of substrate the molecular oxygen production is increased. Alas, the investigative question cannot be fully answered; this is because more tests could have been made with higher concentration of substrate, showing a more accurate representation of what might happen, and, possibly, showing when increase the concentration the reaction rate would no longer increase, proving the hypothesis right. In addition, looking at the graph it is noticeable how the misplay bars are ample; the reason for vast misplay bars could be caused by interrupting the experimental process by moving the table while the experiment was ongoing, inaccurate measuring rod of catalase enzyme, or cleanliness.
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